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cd43 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd43 antibody, anti-human, reafinity
    Cd43 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd43/CD43+Antibody%2C+anti-human%2C+REAfinity/custom%40130-114-749%4041168363
    Average 93 stars, based on 3 article reviews
    cd43 antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Suspension:

    Article Title: Distinct Plasmablast developmental intermediates produce graded expression of IgM secretory transcripts
    Article Snippet: .. Briefly, a single cell suspension of spleen cells was treated with Tris buffered ammonium chloride to lyse red blood cells and then a negative selection, bead-based immunopurification strategy was employed that depletes T, NK, and macrophage cells using a cocktail of biotinylated antibodies specific for CD4, CD8, CD3, CD43, CD49b, Gr1, CD11b, Ter119 (BD Biosciences) and adsorption to Streptavidin microbeads (Miltenyi Biotec Inc.). ..

    Selection:

    Article Title: Distinct Plasmablast developmental intermediates produce graded expression of IgM secretory transcripts
    Article Snippet: .. Briefly, a single cell suspension of spleen cells was treated with Tris buffered ammonium chloride to lyse red blood cells and then a negative selection, bead-based immunopurification strategy was employed that depletes T, NK, and macrophage cells using a cocktail of biotinylated antibodies specific for CD4, CD8, CD3, CD43, CD49b, Gr1, CD11b, Ter119 (BD Biosciences) and adsorption to Streptavidin microbeads (Miltenyi Biotec Inc.). ..

    Article Title: The role of tungsten in modulating DNA double-strand break repair and class switch recombination.
    Article Snippet: .. CD43- splenocytes were isolated by negative selection with CD43-conjugated microbeads and LD magnetic columns (Miltenyi Biotec). .. Cells were labelled with 0.5 μM CFSE (ThermoFisher) and plated at a concentration of 1 × 106 cells/ml in a medium containing 25 ng/ml mouse IL-4 (R&D System) and 50 μg/ml lipopolysaccharide (LPS; Sigma) with or without sodium-, phospho-, or metatungstate.

    Immu-Puri:

    Article Title: Distinct Plasmablast developmental intermediates produce graded expression of IgM secretory transcripts
    Article Snippet: .. Briefly, a single cell suspension of spleen cells was treated with Tris buffered ammonium chloride to lyse red blood cells and then a negative selection, bead-based immunopurification strategy was employed that depletes T, NK, and macrophage cells using a cocktail of biotinylated antibodies specific for CD4, CD8, CD3, CD43, CD49b, Gr1, CD11b, Ter119 (BD Biosciences) and adsorption to Streptavidin microbeads (Miltenyi Biotec Inc.). ..

    Adsorption:

    Article Title: Distinct Plasmablast developmental intermediates produce graded expression of IgM secretory transcripts
    Article Snippet: .. Briefly, a single cell suspension of spleen cells was treated with Tris buffered ammonium chloride to lyse red blood cells and then a negative selection, bead-based immunopurification strategy was employed that depletes T, NK, and macrophage cells using a cocktail of biotinylated antibodies specific for CD4, CD8, CD3, CD43, CD49b, Gr1, CD11b, Ter119 (BD Biosciences) and adsorption to Streptavidin microbeads (Miltenyi Biotec Inc.). ..

    Purification:

    Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling.
    Article Snippet: Viability stains included Zombie Green (BioLegend, 423112), Zombie NIR (BioLegend, 423105), or L/D Fixable Blue (Invitrogen, L34961). .. Splenic B cells were purified as a CD43– fraction using anti-CD43 beads (Miltenyi Biotec, 130-049-801) and cultured at 4 × 106 cells/mL for 18 hours at 37°C with TLR7 agonist R848 (InvivoGen, tlrl-r848-1), TLR9 agonist ODN1668 (InvivoGen, tlrl-1668), or JAK inhibitor tofacitinib (Cell Signaling Technologies, 14703s) at the indicated concentrations. .. Flow cytometry was performed on a Cytek Aurora spectral flow cytometer (Cytek Biosciences), and data analyzed with FlowJo software version 10.8.1or CellEngine (CellCarta).

    Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling.
    Article Snippet: .. Splenic B cells were purified as a CD43– fraction using anti-CD43 beads and subsequently depleted of ABCs using anti-CD11c beads (Miltenyi Biotec, 130-125-835). .. Splenic B cells were purified as a CD43– fraction using anti-CD43 beads (Miltenyi Biotec, 130-049-801) and cultured at 4 × 106 cells/mL for 18 hours at 37°C with TLR7 agonist R848 (InvivoGen, tlrl-r848-1), TLR9 agonist ODN1668 (InvivoGen, tlrl-1668), or JAK inhibitor tofacitinib (Cell Signaling Technologies, 14703s) at the indicated concentrations.

    Cell Culture:

    Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling.
    Article Snippet: Viability stains included Zombie Green (BioLegend, 423112), Zombie NIR (BioLegend, 423105), or L/D Fixable Blue (Invitrogen, L34961). .. Splenic B cells were purified as a CD43– fraction using anti-CD43 beads (Miltenyi Biotec, 130-049-801) and cultured at 4 × 106 cells/mL for 18 hours at 37°C with TLR7 agonist R848 (InvivoGen, tlrl-r848-1), TLR9 agonist ODN1668 (InvivoGen, tlrl-1668), or JAK inhibitor tofacitinib (Cell Signaling Technologies, 14703s) at the indicated concentrations. .. Flow cytometry was performed on a Cytek Aurora spectral flow cytometer (Cytek Biosciences), and data analyzed with FlowJo software version 10.8.1or CellEngine (CellCarta).

    other:

    Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling.
    Article Snippet: Anti-pSTAT3 antibody (Cell Signaling Technology, 9145S) and secondary HRP conjugated anti-rabbit IgG goat antibody (Cell Signaling Technology, 7074S) were used to detect pSTAT3.

    Article Title: Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signaling
    Article Snippet: The proteins on the membrane were detected using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific, PI34577) and imaged using Sapphire Biomolecular Imager (Azure Biosystems).

    Article Title: Cytokine gene expression and wound healing potential of macrophages derived from rat red bone marrow and peripheral blood
    Article Snippet: Далее стёкла вновь промывали три раза в 1× PBS для удаления остатков параформальдегида и наносили первичные антитела в разведении 1:200 в 1× PBS: к CD68 (ab125212, Abcam, Великобритания), CD43 (#130-133-013, Miltenyi Biotec, Германия) и к маркеру гемопоэтических стволовых клеток — CD34 (#PAB18289, Abnova, Тайвань).

    Isolation:

    Article Title: The role of tungsten in modulating DNA double-strand break repair and class switch recombination.
    Article Snippet: .. CD43- splenocytes were isolated by negative selection with CD43-conjugated microbeads and LD magnetic columns (Miltenyi Biotec). .. Cells were labelled with 0.5 μM CFSE (ThermoFisher) and plated at a concentration of 1 × 106 cells/ml in a medium containing 25 ng/ml mouse IL-4 (R&D System) and 50 μg/ml lipopolysaccharide (LPS; Sigma) with or without sodium-, phospho-, or metatungstate.

    Single Cell:

    Article Title: Distinct Plasmablast developmental intermediates produce graded expression of IgM secretory transcripts
    Article Snippet: .. Briefly, a single cell suspension of spleen cells was treated with Tris buffered ammonium chloride to lyse red blood cells and then a negative selection, bead-based immunopurification strategy was employed that depletes T, NK, and macrophage cells using a cocktail of biotinylated antibodies specific for CD4, CD8, CD3, CD43, CD49b, Gr1, CD11b, Ter119 (BD Biosciences) and adsorption to Streptavidin microbeads (Miltenyi Biotec Inc.). ..



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